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| カタログ番号 | HP395012 |
|---|---|
| 説明 |
Recombinant Human CRBN Protein, N-His (HP395012) expressed in E. coli, spanning Met1-Leu442. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96SW2 |
| タンパク質長 | Met1-Leu442 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 52.71 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Protein cereblon, CRBN, AD-006 |
| 背景 | Protein cereblon (CRBN) is a ~50 kDa protein. Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism. 1. Nguyen, TV. et al. (2016) Molecular cell 61, 809-20. PMID: 26990986 2. Lian, Q. et al. (2020) The protein journal 39, 411-421. PMID: 33009960 3. Ito, T. et al. (2010) Science (New York, N.Y.) 327, 1345-50. PMID: 20223979 4. Gandhi, AK. et al. (2014) British journal of haematology 164, 811-21. PMID: 24328678 5. Fischer, ES. et al. (2014) Nature 512, 49-53. PMID: 25043012 6. Chamberlain, PP. et al. (2014) Nature structural & molecular biology 21, 803-9. PMID: 25108355 7. Higgins, JJ. et al. (2008) Neurogenetics 9, 219-23. PMID: 18414909 8. Choi, TY. et al. (2018) The Journal of neuroscience : the official journal of the Society for Neuroscience 38, 3571-3583. PMID: 29530986 9. Kim, MJ. et al. (2019) Frontiers in immunology 10, 2203. PMID: 31620128 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CRBN Protein, N-His
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