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| カタログ番号 | HC301012 |
|---|---|
| 説明 |
Recombinant Human CUL2 Protein, N-His (HC301012) expressed in E. coli, spanning Thr526-Ala745. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13617 |
| タンパク質長 | Thr526-Ala745 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 27.89 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Cullin-2, CUL2, CUL-2 |
| 背景 | Cullin-2 (CUL2) is a ~86 kDa protein. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the E2 ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). 1. Kamura, T. et al. (2001) The Journal of biological chemistry 276, 29748-53. PMID: 11384984 2. Lin, HC. et al. (2015) Science (New York, N.Y.) 349, 91-5. PMID: 26138980 3. Lin, HC. et al. (2018) Molecular cell 70, 602-613.e3. PMID: 29775578 4. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948 5. Zhao, S. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2308870120. PMID: 37844242 6. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650 7. Kamura, T. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 10430-5. PMID: 10973499 8. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982 9. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CUL2 Protein, N-His
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