Anti-Human CUL2 Polyclonal Antibody (HC301014)

Name: Anti-Human CUL2 Polyclonal Antibody
Brand: abinScience
SKU: HC301014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC301014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUL2 (Thr526-Ala745).

Target

Cullin-2, CUL2, CUL-2

Purification

Purified by antigen affinity column.

Accession

Q13617

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cullin-2 (CUL2) is a ~86 kDa protein. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the E2 ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

1. Kamura, T. et al. (2001) The Journal of biological chemistry 276, 29748-53. PMID: 11384984

2. Lin, HC. et al. (2015) Science (New York, N.Y.) 349, 91-5. PMID: 26138980

3. Lin, HC. et al. (2018) Molecular cell 70, 602-613.e3. PMID: 29775578

4. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948

5. Zhao, S. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2308870120. PMID: 37844242

6. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650

7. Kamura, T. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 10430-5. PMID: 10973499

8. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982

9. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

Note

For research use only.

Images

References

Datasheet

Anti-Human CUL2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CUL2 Polyclonal Antibody (HC301014)

Overview

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Datasheet

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