中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HY444012 |
|---|---|
| 説明 |
Recombinant Human CYP17A1 Protein, N-His (HY444012) expressed in E. coli, spanning Cys183-Thr508. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P05093 |
| タンパク質長 | Cys183-Thr508 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 39.22 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Steroid 17-alpha-hydroxylase/17, 20 lyase, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Steroid 17-alpha-monooxygenase, 17-alpha-hydroxyprogesterone aldolase, CYP17A1, S17AH, Cytochrome P450c17, CYP17 |
| 背景 | Steroid 17-alpha-hydroxylase/17,20 lyase (CYP17A1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. 1. DeVore, NM. et al. (2012) Nature 482, 116-9. PMID: 22266943 2. Petrunak, EM. et al. (2014) The Journal of biological chemistry 289, 32952-64. PMID: 25301938 3. Yoshimoto, FK. et al. (2016) The Journal of biological chemistry 291, 17143-64. PMID: 27339894 4. Auchus, RJ. et al. (1998) The Journal of biological chemistry 273, 3158-65. PMID: 9452426 5. Lee, SG. et al. (2023) Journal of inorganic biochemistry 240, 112085. PMID: 36640554 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CYP17A1 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
