

| Numéro de catalogue | HV163022 |
|---|---|
| Description |
Recombinant Human DBR1 Protein, N-His (HV163022) expressed in E. coli, spanning Met1-Pro273. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q9UK59 |
| Longueur de protéine | Met1-Pro273 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Masse moléculaire prédite | 33.44 kDa |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | DBR1, EC:3.1.4.-, Lariat debranching enzyme |
| Contexte | Lariat debranching enzyme is a ~61 kDa protein. Cleaves the 2'-5' phosphodiester linkage at the branch point of excised lariat intron RNA and converts them into linear molecules that can be subsequently degraded, thereby facilitating ribonucleotide turnover. Linked to its role in pre-mRNA processing mechanism, may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis and have an antiviral cell-intrinsic defense function in the brainstem. 1. Kim, JW. et al. (2000) Nucleic acids research 28, 3666-73. PMID: 10982890 2. Ye, Y. et al. (2005) Retrovirology 2, 63. PMID: 16232320 3. Arenas, J. et al. (1987) The Journal of biological chemistry 262, 4274-9. PMID: 2435736 4. Zhang, SY. et al. (2018) Cell 172, 952-965.e18. PMID: 29474921 |
| Note | For research use only |
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