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Anti-Human DBR1 Polyclonal Antibody (HV163014)

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Overview
Catalog No.HV163014
Description
Anti-Human DBR1 Polyclonal Antibody (HV163014) is a rabbit polyclonal antibody detecting DBR1. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human DBR1 (Met1-Pro273).
Target DBR1, EC:3.1.4.-, Lariat debranching enzyme
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9UK59
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Lariat debranching enzyme (DBR1) is a ~61 kDa protein. Cleaves the 2'-5' phosphodiester linkage at the branch point of excised lariat intron RNA and converts them into linear molecules that can be subsequently degraded, thereby facilitating ribonucleotide turnover. Linked to its role in pre-mRNA processing mechanism, may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis and have an antiviral cell-intrinsic defense function in the brainstem.

1. Kim, JW. et al. (2000) Nucleic acids research 28, 3666-73. PMID: 10982890
2. Ye, Y. et al. (2005) Retrovirology 2, 63. PMID: 16232320
3. Arenas, J. et al. (1987) The Journal of biological chemistry 262, 4274-9. PMID: 2435736
4. Zhang, SY. et al. (2018) Cell 172, 952-965.e18. PMID: 29474921
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human DBR1 Polyclonal Antibody

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