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| カタログ番号 | HV163022 |
|---|---|
| 説明 |
Recombinant Human DBR1 Protein, N-His (HV163022) expressed in E. coli, spanning Met1-Pro273. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UK59 |
| タンパク質長 | Met1-Pro273 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DBR1, EC:3.1.4.-, Lariat debranching enzyme |
| 背景 | Lariat debranching enzyme is a ~61 kDa protein. Cleaves the 2'-5' phosphodiester linkage at the branch point of excised lariat intron RNA and converts them into linear molecules that can be subsequently degraded, thereby facilitating ribonucleotide turnover. Linked to its role in pre-mRNA processing mechanism, may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis and have an antiviral cell-intrinsic defense function in the brainstem. 1. Kim, JW. et al. (2000) Nucleic acids research 28, 3666-73. PMID: 10982890 2. Ye, Y. et al. (2005) Retrovirology 2, 63. PMID: 16232320 3. Arenas, J. et al. (1987) The Journal of biological chemistry 262, 4274-9. PMID: 2435736 4. Zhang, SY. et al. (2018) Cell 172, 952-965.e18. PMID: 29474921 |
| 注意事項 | For research use only |
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