Recombinant Human DDB1 Protein, C-His (HS873011) expressed in Insect Cells, spanning Met1-His1140. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión
Insect cells
Número de acceso
Q16531
Longitud de proteína
Met1-His1140
Aplicaciones
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
127.93 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
XAP-1, XPE-binding factor, DNA damage-binding protein a, XAP1, Xeroderma pigmentosum group E-complementing protein, DDB1, XPCe, DDBa, XPE-BF, DDB p127 subunit, Damage-specific DNA-binding protein 1, UV-DDB 1, UV-damaged DNA-binding protein 1, UV-damaged DNA-binding factor, DNA damage-binding protein 1, HBV X-associated protein 1
Fondo
DNA damage-binding protein 1 (DDB1) is a ~126 kDa protein. Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1.
1. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464
2. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697
3. Kulaksiz, G. et al. (2005) Molecular and cellular biology 25, 9784-92. PMID: 16260596
4. Hu, J. et al. (2006) The Journal of biological chemistry 281, 3753-6. PMID: 16407242
5. Senga, T. et al. (2006) The Journal of biological chemistry 281, 6246-52. PMID: 16407252
6. Nishitani, H. et al. (2006) The EMBO journal 25, 1126-36. PMID: 16482215
7. Lovejoy, CA. et al. (2006) Molecular and cellular biology 26, 7977-90. PMID: 16940174
8. He, YJ. et al. (2006) Genes & development 20, 2949-54. PMID: 17079684
9. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human DDB1 Protein, C-His
中文
English
한국어
日本語
Español
Français
Русский
