Recombinant Human DDB1 Protein, C-His (HS873011) expressed in Insect Cells, spanning Met1-His1140. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Insect cells
Numéro d'accès
Q16531
Longueur de protéine
Met1-His1140
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
127.93 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
XAP-1, XPE-binding factor, DNA damage-binding protein a, XAP1, Xeroderma pigmentosum group E-complementing protein, DDB1, XPCe, DDBa, XPE-BF, DDB p127 subunit, Damage-specific DNA-binding protein 1, UV-DDB 1, UV-damaged DNA-binding protein 1, UV-damaged DNA-binding factor, DNA damage-binding protein 1, HBV X-associated protein 1
Contexte
DNA damage-binding protein 1 (DDB1) is a ~126 kDa protein. Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1.
1. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464
2. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697
3. Kulaksiz, G. et al. (2005) Molecular and cellular biology 25, 9784-92. PMID: 16260596
4. Hu, J. et al. (2006) The Journal of biological chemistry 281, 3753-6. PMID: 16407242
5. Senga, T. et al. (2006) The Journal of biological chemistry 281, 6246-52. PMID: 16407252
6. Nishitani, H. et al. (2006) The EMBO journal 25, 1126-36. PMID: 16482215
7. Lovejoy, CA. et al. (2006) Molecular and cellular biology 26, 7977-90. PMID: 16940174
8. He, YJ. et al. (2006) Genes & development 20, 2949-54. PMID: 17079684
9. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human DDB1 Protein, C-His
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