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Обзор
Каталожный номерHY134022
Описание
Recombinant Human DEFA4 Protein, N-GST & C-His (HY134022) expressed in E. coli, spanning Gly20-Asp97. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP12838
Длина белкаGly20-Asp97
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 36.65 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияCorticostatin HP-4, DEF4, DEFA4, Defensin alpha 4, HNP-4, HP-4, Neutrophil defensin 4
Фон

Defensin alpha 4 is a ~10 kDa protein. Host-defense peptide that has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Exhibits antimicrobial activity against Gram-negative E.coli and E.aerogenes and Gram-positive S.faecalis, S.aureus and B.cereus and the yeast C.albicans (in vitro). Interacts with pathogenic surface proteins and toxins, such as HIV-1 surface protein gp120 and B.anthracis anthrax lethal factor lef. Protects blood cells against infection with HIV-1 (in vitro). Inhibits enzymatic activity of B.anthracis lef/anthrax lethal factor (in vitro).

1. Wu, Z. et al. (2004) The journal of peptide research : official journal of the American Peptide Society 64, 118-25. PMID: 15317502
2. Ericksen, B. et al. (2005) Antimicrobial agents and chemotherapy 49, 269-75. PMID: 15616305
3. Wilde, CG. et al. (1989) The Journal of biological chemistry 264, 11200-3. PMID: 2500436
4. Gabay, JE. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5610-4. PMID: 2501794
5. Hu, H. et al. (2019) Biochimica et biophysica acta. Biomembranes 1861, 835-844. PMID: 30658057
6. Szyk, A. et al. (2006) Protein science : a publication of the Protein Society 15, 2749-60. PMID: 17088326
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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