Human DEFA4 Recombinant Protein (N-GST) (HY134012)

Name: Human DEFA4 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HY134012
Price: 211 USD
Availability: InStock

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Catalog No.	HY134012
Description	Recombinant Human DEFA4 Protein, N-GST (HY134012) expressed in E. coli, spanning Gly20-Asp97. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P12838
Protein length	Gly20-Asp97
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.3 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DEF4, Defensin, alpha 4, HP-4, HNP-4, Neutrophil defensin 4, DEFA4
Background	Defensin alpha 4 (DEFA4) is a ~10 kDa protein. Host-defense peptide that has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Exhibits antimicrobial activity against Gram-negative E.coli and E.aerogenes and Gram-positive S.faecalis, S.aureus and B.cereus and the yeast C.albicans (in vitro). Interacts with pathogenic surface proteins and toxins, such as HIV-1 surface protein gp120 and B.anthracis anthrax lethal factor lef. Protects blood cells against infection with HIV-1 (in vitro). Inhibits enzymatic activity of B.anthracis lef/anthrax lethal factor (in vitro). 1. Wu, Z. et al. (2004) The journal of peptide research : official journal of the American Peptide Society 64, 118-25. PMID: 15317502 2. Ericksen, B. et al. (2005) Antimicrobial agents and chemotherapy 49, 269-75. PMID: 15616305 3. Wilde, CG. et al. (1989) The Journal of biological chemistry 264, 11200-3. PMID: 2500436 4. Gabay, JE. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5610-4. PMID: 2501794 5. Hu, H. et al. (2019) Biochimica et biophysica acta. Biomembranes 1861, 835-844. PMID: 30658057 6. Szyk, A. et al. (2006) Protein science : a publication of the Protein Society 15, 2749-60. PMID: 17088326
Note	For research use only.

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SDS-PAGE for Recombinant Human DEFA4 Protein, N-GST

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Datasheet

Human DEFA4 Recombinant Protein (N-GST).pdf

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Human DEFA4 Recombinant Protein (N-GST) (HY134012)

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