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Обзор
Каталожный номерHX187032
Описание
Recombinant Human DEFA5 Protein, N-GST & C-His (HX187032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ01523
Длина белкаGlu20-Arg94
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияDEF5, DEFA5, Defensin alpha 5, Defensin-5, HD5(20-94), HD5(23-94), HD5(29-94), HD5(56-94), HD5(63-94)
Фон

Defensin alpha 5 is a ~10 kDa protein. Host-defense peptide that maintains sterility in the urogenital system. Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes. Confers resistance to intestinal infection by S.typhimurium. Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus. Binds to bacterial membranes and causes membrane disintegration.

1. Ghosh, D. et al. (2002) Nature immunology 3, 583-90. PMID: 12021776
2. Salzman, NH. et al. (2003) Nature 422, 522-6. PMID: 12660734
3. Ericksen, B. et al. (2005) Antimicrobial agents and chemotherapy 49, 269-75. PMID: 15616305
4. de Leeuw, E. et al. (2009) FEBS letters 583, 2507-12. PMID: 19589339
5. Spencer, JD. et al. (2012) PloS one 7, e31712. PMID: 22359618
6. Rajabi, M. et al. (2012) The Journal of biological chemistry 287, 21615-27. PMID: 22573326
7. Schroeder, BO. et al. (2015) Mucosal immunology 8, 661-71. PMID: 25354318
8. Wang, C. et al. (2015) Journal of medicinal chemistry 58, 3083-93. PMID: 25782105
9. Ehmann, D. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 3746-3751. PMID: 30808760
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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