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Human DEPTOR Recombinant Protein (N-GST & C-His) (HP016022)

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Aperçu
Numéro de catalogueHP016022
Description
Recombinant Human DEPTOR Protein, N-GST & C-His (HP016022) expressed in E. coli, spanning Ser316-Cys409. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ8TB45
Longueur de protéineSer316-Cys409
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 38.20 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsDEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPDC6, DEPTOR, hDEPTOR
Contexte

DEP domain-containing mTOR-interacting protein is a ~46 kDa protein. Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity.

1. Peterson, TR. et al. (2009) Cell 137, 873-86. PMID: 19446321
2. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875
3. Zhao, Y. et al. (2011) Molecular cell 44, 304-16. PMID: 22017876
4. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877
5. Yoon, MS. et al. (2015) Molecular cell 58, 549-56. PMID: 25936805
6. Zhao, L. et al. (2018) The Journal of biological chemistry 293, 4883-4892. PMID: 29382726
7. Wälchli, M. et al. (2021) eLife 10. PMID: 34519268
8. Heimhalt, M. et al. (2021) eLife 10. PMID: 34519269
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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