

| Numéro de catalogue | HP016022 |
|---|---|
| Description |
Recombinant Human DEPTOR Protein, N-GST & C-His (HP016022) expressed in E. coli, spanning Ser316-Cys409. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q8TB45 |
| Longueur de protéine | Ser316-Cys409 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Masse moléculaire prédite | 38.20 kDa |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPDC6, DEPTOR, hDEPTOR |
| Contexte | DEP domain-containing mTOR-interacting protein is a ~46 kDa protein. Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity. 1. Peterson, TR. et al. (2009) Cell 137, 873-86. PMID: 19446321 2. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875 3. Zhao, Y. et al. (2011) Molecular cell 44, 304-16. PMID: 22017876 4. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877 5. Yoon, MS. et al. (2015) Molecular cell 58, 549-56. PMID: 25936805 6. Zhao, L. et al. (2018) The Journal of biological chemistry 293, 4883-4892. PMID: 29382726 7. Wälchli, M. et al. (2021) eLife 10. PMID: 34519268 8. Heimhalt, M. et al. (2021) eLife 10. PMID: 34519269 |
| Note | For research use only |
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