Anti-Human DEPTOR Polyclonal Antibody (HP016014)

Name: Anti-Human DEPTOR Polyclonal Antibody
Brand: abinScience
SKU: HP016014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP016014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DEPTOR (Ser316-Cys409).

Target

DEPDC6, DEP domain-containing protein 6, DEP domain-containing mTOR-interacting protein, DEPTOR

Purification

Purified by antigen affinity column.

Accession

Q8TB45

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DEP domain-containing mTOR-interacting protein (DEPTOR) is a ~46 kDa protein. Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity.

1. Peterson, TR. et al. (2009) Cell 137, 873-86. PMID: 19446321

2. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875

3. Zhao, Y. et al. (2011) Molecular cell 44, 304-16. PMID: 22017876

4. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877

5. Yoon, MS. et al. (2015) Molecular cell 58, 549-56. PMID: 25936805

6. Zhao, L. et al. (2018) The Journal of biological chemistry 293, 4883-4892. PMID: 29382726

7. Wälchli, M. et al. (2021) eLife 10. PMID: 34519268

8. Heimhalt, M. et al. (2021) eLife 10. PMID: 34519269

Note

For research use only.

Images

References

Datasheet

Anti-Human DEPTOR Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human DEPTOR Polyclonal Antibody (HP016014)

Overview

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References

Datasheet

SDS

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