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| カタログ番号 | HV555012 |
|---|---|
| 説明 |
Recombinant Human DPP9 Protein, N-His (HV555012) expressed in E. coli, spanning Ser36-Ser202. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q86TI2 |
| タンパク質長 | Ser36-Ser202 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.4 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DPRP-2, DPLP9, Dipeptidyl peptidase IV-related protein 2, DPP IX, DPP9, Dipeptidyl peptidase-like protein 9, DP9, DPRP2 |
| 背景 | Dipeptidyl peptidase 9 (DPP9) is a ~98 kDa protein. Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP9, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition. 1. Qi, SY. et al. (2003) The Biochemical journal 373, 179-89. PMID: 12662155 2. Bjelke, JR. et al. (2006) The Biochemical journal 396, 391-9. PMID: 16475979 3. Geiss-Friedlander, R. et al. (2009) The Journal of biological chemistry 284, 27211-9. PMID: 19667070 4. Ross, B. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1437-E1445. PMID: 29382749 5. Zhong, FL. et al. (2018) The Journal of biological chemistry 293, 18864-18878. PMID: 30291141 6. Huang, M. et al. (2021) Nature 592, 773-777. PMID: 33731929 7. Harapas, CR. et al. (2022) Science immunology 7, eabi4611. PMID: 36112693 8. Okondo, MC. et al. (2017) Nature chemical biology 13, 46-53. PMID: 27820798 9. Johnson, DC. et al. (2018) Nature medicine 24, 1151-1156. PMID: 29967349 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human DPP9 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
