Anti-DPP9 Polyclonal Antibody (HV555014)

Name: Anti-DPP9 Polyclonal Antibody
Brand: abinScience
SKU: HV555014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HV555014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DPP9 (Ser36-Ser202).

Target

Dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DPRP-2, DPLP9, Dipeptidyl peptidase IV-related protein 2, DPP IX, DPP9, Dipeptidyl peptidase-like protein 9, DP9, DPRP2

Purification

Purified by antigen affinity column.

Accession

Q86TI2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dipeptidyl peptidase 9 (DPP9) is a ~98 kDa protein. Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP9, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition.

1. Qi, SY. et al. (2003) The Biochemical journal 373, 179-89. PMID: 12662155

2. Bjelke, JR. et al. (2006) The Biochemical journal 396, 391-9. PMID: 16475979

3. Geiss-Friedlander, R. et al. (2009) The Journal of biological chemistry 284, 27211-9. PMID: 19667070

4. Ross, B. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1437-E1445. PMID: 29382749

5. Zhong, FL. et al. (2018) The Journal of biological chemistry 293, 18864-18878. PMID: 30291141

6. Huang, M. et al. (2021) Nature 592, 773-777. PMID: 33731929

7. Harapas, CR. et al. (2022) Science immunology 7, eabi4611. PMID: 36112693

8. Okondo, MC. et al. (2017) Nature chemical biology 13, 46-53. PMID: 27820798

9. Johnson, DC. et al. (2018) Nature medicine 24, 1151-1156. PMID: 29967349

Note

For research use only.

Images

References

Datasheet

Anti-DPP9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-DPP9 Polyclonal Antibody (HV555014)

Overview

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Datasheet

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