Human EFEMP2 Recombinant Protein (N-GST) (HF514012)

Name: Human EFEMP2 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HF514012
Price: 211 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue	HF514012
Description	Recombinant Human EFEMP2 Protein, N-GST (HF514012) expressed in E. coli, spanning Ser26-Phe443. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O95967
Longueur de protéine	Ser26-Phe443
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	73.66 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	EGF-containing fibulin-like extracellular matrix protein 2, Protein UPH1, Fibulin-4, FBLN4, EFEMP2, FIBL-4
Contexte	EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) is a ~49 kDa protein. Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta. In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin. Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction. Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling. 1. Sasaki, T. et al. (2016) Matrix biology : journal of the International Society for Matrix Biology 56, 132-149. PMID: 27339457 2. Cirulis, JT. et al. (2008) Biochemistry 47, 12601-13. PMID: 18973305 3. Choudhury, R. et al. (2009) The Journal of biological chemistry 284, 24553-67. PMID: 19570982 4. Djokic, J. et al. (2013) The Journal of biological chemistry 288, 22821-35. PMID: 23782690
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human EFEMP2 Protein, N-GST

Reférences

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Human EFEMP2 Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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