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| カタログ番号 | HB920012 |
|---|---|
| 説明 |
Recombinant Human ENPP1 Protein, N-His (HB920012) expressed in E. coli, spanning Asp362-Trp518. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P22413 |
| タンパク質長 | Asp362-Trp518 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.6 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, NPPS, PC1, NPPase, PDNP1, Membrane component chromosome 6 surface marker 1, ENPP1, Phosphodiesterase I/nucleotide pyrophosphatase 1, M6S1, Plasma-cell membrane glycoprotein PC-1, E-NPP 1, Nucleotide diphosphatase |
| 背景 | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) is a ~104 kDa protein. Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect. 1. Johnson, KA. et al. (2000) American journal of physiology. Regulatory, integrative and comparative physiology 279, R1365-77. PMID: 11004006 2. Li, L. et al. (2014) Nature chemical biology 10, 1043-8. PMID: 25344812 3. Stella, J. et al. (2016) Human mutation 37, 1190-1201. PMID: 27467858 4. Namasivayam, V. et al. (2017) Biochimica et biophysica acta. General subjects 1861, 603-614. PMID: 28011303 5. Szeri, F. et al. (2022) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37, 1024-1031. PMID: 35147247 6. Belli, SI. et al. (1994) European journal of biochemistry 226, 433-43. PMID: 8001561 7. Maddux, BA. et al. (2000) Diabetes 49, 13-9. PMID: 10615944 8. Chourabi, M. et al. (2018) The Journal of investigative dermatology 138, 291-300. PMID: 28964717 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ENPP1 Protein, N-His
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