Anti-Human ENPP1 Polyclonal Antibody (HB920014)

Name: Anti-Human ENPP1 Polyclonal Antibody
Brand: abinScience
SKU: HB920014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB920014

Description

Anti-Human ENPP1 Polyclonal Antibody (HB920014) is a rabbit polyclonal antibody detecting ENPP1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ENPP1 (Asp362-Trp518).

Target

ENPP1

Purification

Purified by antigen affinity column.

Accession

P22413

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, 3.1.4.1, Nucleotide pyrophosphatase, NPPase, 3.6.1.9, Nucleotide diphosphatase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form, ENPP1, M6S1, NPPS, PC1, PDNP1

Background

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) is a ~104 kDa protein. Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect.

1. Johnson, KA. et al. (2000) American journal of physiology. Regulatory, integrative and comparative physiology 279, R1365-77. PMID: 11004006

2. Li, L. et al. (2014) Nature chemical biology 10, 1043-8. PMID: 25344812

3. Stella, J. et al. (2016) Human mutation 37, 1190-1201. PMID: 27467858

4. Namasivayam, V. et al. (2017) Biochimica et biophysica acta. General subjects 1861, 603-614. PMID: 28011303

5. Szeri, F. et al. (2022) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37, 1024-1031. PMID: 35147247

6. Belli, SI. et al. (1994) European journal of biochemistry 226, 433-43. PMID: 8001561

7. Maddux, BA. et al. (2000) Diabetes 49, 13-9. PMID: 10615944

8. Chourabi, M. et al. (2018) The Journal of investigative dermatology 138, 291-300. PMID: 28964717

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ENPP1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-Human ENPP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ENPP1 Polyclonal Antibody (HB920014)

Overview

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References

Datasheet

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