Human ETF1 Recombinant Protein (N-His) (HD118012)

Name: Human ETF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD118012
Price: 211 USD
Availability: InStock

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Número de catálogo	HD118012
Descripción	Recombinant Human ETF1 Protein, N-His (HD118012) expressed in E. coli, spanning Met1-Ser257. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P62495
Longitud de proteína	Met1-Ser257
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	30.52 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1
Fondo	Eukaryotic peptide chain release factor subunit 1 is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. 1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813 2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602 3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019 4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381 5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242 6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793 7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965 8. Yamashita, A. et al. (2009) Genes & development 23, 1091-105. PMID: 19417104 9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371
Nota	For research use only

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Datasheet

Human ETF1 Recombinant Protein (N-His).pdf

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