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Anti-Human ETF1 Polyclonal Antibody (HD118014)

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Overview
Catalog No.HD118014
Description
Anti-Human ETF1 Polyclonal Antibody (HD118014) is a rabbit polyclonal antibody detecting ETF1. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ETF1 (Met1-Ser257).
Target ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P62495
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Eukaryotic peptide chain release factor subunit 1 (ETF1) is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813
2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602
3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019
4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381
5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242
6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793
7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965
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9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371
Note For research use only
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Datasheet
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Formula
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Anti-Human ETF1 Polyclonal Antibody

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