Human FANCM Recombinant Protein (N-His) (HA265012)

Name: Human FANCM Recombinant Protein (N-His)
Brand: abinScience
SKU: HA265012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HA265012
Descripción	Recombinant Human FANCM Protein, N-His (HA265012) expressed in E. coli, spanning Ser281-His620. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q8IYD8
Longitud de proteína	Ser281-His620
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	41.44 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Fanconi anemia group M protein, Protein FACM, 3.6.4.13, ATP-dependent RNA helicase FANCM, Fanconi anemia-associated polypeptide of 250 kDa, FAAP250, Protein Hef ortholog, FANCM, KIAA1596
Fondo	Fanconi anemia group M protein (FANCM) is a ~232 kDa protein. DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. 1. Meetei, AR. et al. (2005) Nature genetics 37, 958-63. PMID: 16116422 2. Singh, TR. et al. (2009) Blood 114, 174-80. PMID: 19423727 3. Yan, Z. et al. (2010) Molecular cell 37, 865-78. PMID: 20347428 4. Singh, TR. et al. (2010) Molecular cell 37, 879-86. PMID: 20347429 5. Fouquet, B. et al. (2017) eLife 6. PMID: 29231814 6. Ciccia, A. et al. (2007) Molecular cell 25, 331-43. PMID: 17289582
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human FANCM Protein, N-His

Referencias

Datasheet

Human FANCM Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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