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| カタログ番号 | HP707012 |
|---|---|
| 説明 |
Recombinant Human FLCN Protein, N-His (HP707012) expressed in E. coli, spanning Lys347-Asn579. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8NFG4 |
| タンパク質長 | Lys347-Asn579 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.46 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BHD skin lesion fibrofolliculoma protein, FLCN, Birt-Hogg-Dube syndrome protein, BHD, Folliculin |
| 背景 | Folliculin (FLCN) is a ~64 kDa protein. Multi-functional protein, involved in both the cellular response to amino acid availability and in the regulation of glycolysis. GTPase-activating protein that plays a key role in the cellular response to amino acid availability through regulation of the non-canonical mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3. Activates mTORC1 by acting as a GTPase-activating protein: specifically stimulates GTP hydrolysis by RagC/RRAGC or RagD/RRAGD, promoting the conversion to the GDP-bound state of RagC/RRAGC or RagD/RRAGD, and thereby activating the kinase activity of mTORC1. The GTPase-activating activity is inhibited during starvation and activated in presence of nutrients. Acts as a key component for non-canonical mTORC1-dependent control of the MiT/TFE factors TFEB and TFE3, while it is not involved in mTORC1-dependent phosphorylation of canonical RPS6KB1/S6K1 and EIF4EBP1/4E-BP1. 1. Baba, M. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 15552-7. PMID: 17028174 2. Takagi, Y. et al. (2008) Oncogene 27, 5339-47. PMID: 18663353 3. Hong, SB. et al. (2010) PloS one 5, e15793. PMID: 21209915 4. Petit, CS. et al. (2013) The Journal of cell biology 202, 1107-22. PMID: 24081491 5. Tsun, ZY. et al. (2013) Molecular cell 52, 495-505. PMID: 24095279 6. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913 7. Shen, K. et al. (2019) Cell 179, 1319-1329.e8. PMID: 31704029 8. Napolitano, G. et al. (2020) Nature 585, 597-602. PMID: 32612235 9. Woodford, MR. et al. (2021) Nature structural & molecular biology 28, 662-670. PMID: 34381247 10. Jansen, RM. et al. (2022) Science advances 8, eadd2926. PMID: 36103527 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human FLCN Protein, N-His
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