Anti-Human FLCN Polyclonal Antibody (HP707014)

Name: Anti-Human FLCN Polyclonal Antibody
Brand: abinScience
SKU: HP707014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HP707014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human FLCN (Lys347-Asn579).

Target

BHD skin lesion fibrofolliculoma protein, FLCN, Birt-Hogg-Dube syndrome protein, BHD, Folliculin

Purification

Purified by antigen affinity column.

Accession

Q8NFG4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Folliculin (FLCN) is a ~64 kDa protein. Multi-functional protein, involved in both the cellular response to amino acid availability and in the regulation of glycolysis. GTPase-activating protein that plays a key role in the cellular response to amino acid availability through regulation of the non-canonical mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3. Activates mTORC1 by acting as a GTPase-activating protein: specifically stimulates GTP hydrolysis by RagC/RRAGC or RagD/RRAGD, promoting the conversion to the GDP-bound state of RagC/RRAGC or RagD/RRAGD, and thereby activating the kinase activity of mTORC1. The GTPase-activating activity is inhibited during starvation and activated in presence of nutrients. Acts as a key component for non-canonical mTORC1-dependent control of the MiT/TFE factors TFEB and TFE3, while it is not involved in mTORC1-dependent phosphorylation of canonical RPS6KB1/S6K1 and EIF4EBP1/4E-BP1.

1. Baba, M. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 15552-7. PMID: 17028174

2. Takagi, Y. et al. (2008) Oncogene 27, 5339-47. PMID: 18663353

3. Hong, SB. et al. (2010) PloS one 5, e15793. PMID: 21209915

4. Petit, CS. et al. (2013) The Journal of cell biology 202, 1107-22. PMID: 24081491

5. Tsun, ZY. et al. (2013) Molecular cell 52, 495-505. PMID: 24095279

6. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913

7. Shen, K. et al. (2019) Cell 179, 1319-1329.e8. PMID: 31704029

8. Napolitano, G. et al. (2020) Nature 585, 597-602. PMID: 32612235

9. Woodford, MR. et al. (2021) Nature structural & molecular biology 28, 662-670. PMID: 34381247

10. Jansen, RM. et al. (2022) Science advances 8, eadd2926. PMID: 36103527

Note

For research use only.

Images

References

Datasheet

Anti-Human FLCN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human FLCN Polyclonal Antibody (HP707014)

Overview

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Datasheet

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