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Human FOXP1 Recombinant Protein (N-His) (HV703012)

Human FOXP1 Recombinant Protein (N-His)
Human FOXP1 Recombinant Protein (N-His)
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Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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Vista general
Número de catálogoHV703012
Descripción
Recombinant Human FOXP1 Protein, N-His (HV703012) expressed in E. coli, spanning Asn461-Pro671. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ9H334
Longitud de proteínaAsn461-Pro671
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 26.03 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosFOXP1, MFH, Forkhead box protein P1, Mac-1-regulated forkhead
Fondo

Forkhead box protein P1 (FOXP1) is a ~75 kDa protein. Transcriptional repressor. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Plays an important role in the specification and differentiation of lung epithelium. Acts cooperatively with FOXP4 to regulate lung secretory epithelial cell fate and regeneration by restricting the goblet cell lineage program; the function may involve regulation of AGR2. Essential transcriptional regulator of B-cell development.

1. Jepsen, K. et al. (2008) Genes & development 22, 740-5. PMID: 18347093
2. Sollis, E. et al. (2016) Human molecular genetics 25, 546-57. PMID: 26647308
3. van Keimpema, M. et al. (2014) Blood 124, 3431-40. PMID: 25267198
4. Shi, C. et al. (2004) The Journal of clinical investigation 114, 408-18. PMID: 15286807
5. Shi, C. et al. (2008) Blood 112, 4699-711. PMID: 18799727
6. Grundmann, S. et al. (2013) PloS one 8, e70873. PMID: 24023716
7. Takayama, K. et al. (2008) Biochemical and biophysical research communications 374, 388-93. PMID: 18640093
8. Kamran, M. et al. (2017) Oncogenesis 6, e298. PMID: 28218735
Nota For research use only.
Imágenes
  • Human FOXP1 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human FOXP1 Protein, N-His

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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