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| カタログ番号 | HF008032 |
|---|---|
| 説明 |
Recombinant Human GABARAP Protein, C-His-Avi (HF008032) expressed in E. coli, spanning Met1-Leu117. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O95166 |
| タンパク質長 | Met1-Leu117 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.84 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | MM46, GABA(A) receptor-associated protein, GABARAP, Gamma-aminobutyric acid receptor-associated protein, FLC3B |
| 背景 | Gamma-aminobutyric acid receptor-associated protein (GABARAP) is a ~13 kDa protein. Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation. 1. Wang, H. et al. (1999) Nature 397, 69-72. PMID: 9892355 2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837 3. Behrends, C. et al. (2010) Nature 466, 68-76. PMID: 20562859 4. Colecchia, D. et al. (2012) Autophagy 8, 1724-40. PMID: 22948227 5. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538 6. Genau, HM. et al. (2015) Molecular cell 57, 995-1010. PMID: 25684205 7. Lee, JH. et al. (2005) Biotechnology letters 27, 623-8. PMID: 15977068 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human GABARAP Protein, C-His-Avi
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