Anti-Human GABARAP Polyclonal Antibody (HF008014)

Name: Anti-Human GABARAP Polyclonal Antibody
Brand: abinScience
SKU: HF008014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HF008014

Description

Anti-Human GABARAP Polyclonal Antibody (HF008014) is a rabbit polyclonal antibody detecting GABARAP in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cavia porcellus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GABARAP (Met1-Leu117).

Target

MM46, GABA(A) receptor-associated protein, GABARAP, Gamma-aminobutyric acid receptor-associated protein, FLC3B

Purification

Purified by antigen affinity column.

Accession

O95166

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Gamma-aminobutyric acid receptor-associated protein (GABARAP) is a ~13 kDa protein. Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation.

1. Wang, H. et al. (1999) Nature 397, 69-72. PMID: 9892355

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Behrends, C. et al. (2010) Nature 466, 68-76. PMID: 20562859

4. Colecchia, D. et al. (2012) Autophagy 8, 1724-40. PMID: 22948227

5. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

6. Genau, HM. et al. (2015) Molecular cell 57, 995-1010. PMID: 25684205

7. Lee, JH. et al. (2005) Biotechnology letters 27, 623-8. PMID: 15977068

Note

For research use only.

Images

References

Datasheet

Anti-Human GABARAP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human GABARAP Polyclonal Antibody (HF008014)

Overview

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References

Datasheet

SDS

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