Human GLI1 Recombinant Protein (N-His) (HY371012)

Name: Human GLI1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY371012
Price: 211 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HY371012
Descripción	Recombinant Human GLI1 Protein, N-His (HY371012) expressed in E. coli, spanning Phe2-Glu234. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P08151
Longitud de proteína	Phe2-Glu234
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	27.02 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Oncogene GLI, GLI, Glioma-associated oncogene, GLI1, Zinc finger protein GLI1
Fondo	Zinc finger protein GLI1 is a ~117 kDa protein. Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. 1. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483 2. Lo, HW. et al. (2009) Cancer research 69, 6790-8. PMID: 19706761 3. Maloverjan, A. et al. (2010) Experimental cell research 316, 627-37. PMID: 19878745 4. Gilder, AS. et al. (2013) Biochemical and biophysical research communications 440, 431-6. PMID: 24076122 5. Zhang, Y. et al. (2013) Nature communications 4, 2608. PMID: 24217340 6. Cherry, AL. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2563-79. PMID: 24311597 7. Kinzler, KW. et al. (1990) Molecular and cellular biology 10, 634-42. PMID: 2105456 8. Pavletich, NP. et al. (1993) Science (New York, N.Y.) 261, 1701-7. PMID: 8378770
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human GLI1 Protein, N-His

Referencias

Datasheet

Human GLI1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo