Anti-GLI1 Polyclonal Antibody (HY371014)

Name: Anti-GLI1 Polyclonal Antibody
Brand: abinScience
SKU: HY371014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY371014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GLI1 (Phe2-Glu234).

Target

Oncogene GLI, GLI, Glioma-associated oncogene, GLI1, Zinc finger protein GLI1

Purification

Purified by antigen affinity column.

Accession

P08151

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Zinc finger protein GLI1 is a ~117 kDa protein. Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling.

1. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483

2. Lo, HW. et al. (2009) Cancer research 69, 6790-8. PMID: 19706761

3. Maloverjan, A. et al. (2010) Experimental cell research 316, 627-37. PMID: 19878745

4. Gilder, AS. et al. (2013) Biochemical and biophysical research communications 440, 431-6. PMID: 24076122

5. Zhang, Y. et al. (2013) Nature communications 4, 2608. PMID: 24217340

6. Cherry, AL. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2563-79. PMID: 24311597

7. Kinzler, KW. et al. (1990) Molecular and cellular biology 10, 634-42. PMID: 2105456

8. Pavletich, NP. et al. (1993) Science (New York, N.Y.) 261, 1701-7. PMID: 8378770

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GLI1 polyclonal antibody at 1ug/mL on recombinant GLI1 (Catalog No. HY371012).

References

Datasheet

Anti-GLI1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GLI1 Polyclonal Antibody (HY371014)

Overview

Images

References

Datasheet

SDS

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