ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
28.29 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37, GPBAR1, TGR5
Фон
G-protein coupled bile acid receptor 1 (GPBAR1) is a ~35 kDa protein. G protein-coupled receptor for bile acid. Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order. Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation.
1. Maruyama, T. et al. (2002) Biochemical and biophysical research communications 298, 714-9. PMID: 12419312
2. Kawamata, Y. et al. (2003) The Journal of biological chemistry 278, 9435-40. PMID: 12524422
3. Yang, F. et al. (2020) Nature 587, 499-504. PMID: 32698187
4. Chen, G. et al. (2020) Signal transduction and targeted therapy 5, 142. PMID: 32747649
5. Ma, L. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2117054119. PMID: 35858343
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human GPBAR1 Protein, N-His-KSI
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