Anti-Human GPBAR1 Polyclonal Antibody (HA179014)

Name: Anti-Human GPBAR1 Polyclonal Antibody
Brand: abinScience
SKU: HA179014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HA179014

Description

Anti-Human GPBAR1 Polyclonal Antibody (HA179014) is a rabbit polyclonal antibody detecting GPBAR1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human GPBAR1.

Target

G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37, GPBAR1, TGR5

Purification

Purified by antigen affinity column.

Accession

Q8TDU6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

G-protein coupled bile acid receptor 1 (GPBAR1) is a ~35 kDa protein. G protein-coupled receptor for bile acid. Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order. Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation.

1. Maruyama, T. et al. (2002) Biochemical and biophysical research communications 298, 714-9. PMID: 12419312

2. Kawamata, Y. et al. (2003) The Journal of biological chemistry 278, 9435-40. PMID: 12524422

3. Yang, F. et al. (2020) Nature 587, 499-504. PMID: 32698187

4. Chen, G. et al. (2020) Signal transduction and targeted therapy 5, 142. PMID: 32747649

5. Ma, L. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2117054119. PMID: 35858343

Note

For research use only.

Images

References

Datasheet

Anti-Human GPBAR1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GPBAR1 Polyclonal Antibody (HA179014)

Overview

Images

References

Datasheet

SDS

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