

| Каталожный номер | HP876022 |
|---|---|
| Описание |
Recombinant Human GPIHBP1 Protein, N-His-SUMO (HP876022) expressed in E. coli, spanning Ser59-Gln132. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | Q8IV16 |
| Длина белка | Ser59-Gln132 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 20.42 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | GPIHBP1, Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1, GPI-anchored HDL-binding protein 1, HBP1, High density lipoprotein-binding protein 1, GPI-HBP1 |
| Фон | Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1) is a ~19 kDa protein. Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries. Anchors LPL on the surface of endothelial cells in the lumen of blood capillaries. Protects LPL against loss of activity, and against ANGPTL4-mediated unfolding. Thereby, plays an important role in lipolytic processing of chylomicrons by LPL, triglyceride metabolism and lipid homeostasis. Binds chylomicrons and phospholipid particles that contain APOA5. 1. Mysling, S. et al. (2016) eLife 5. PMID: 27929370 2. Kristensen, KK. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6020-E6029. PMID: 29899144 3. Beigneux, AP. et al. (2009) Arteriosclerosis, thrombosis, and vascular biology 29, 956-62. PMID: 19304573 4. Coca-Prieto, I. et al. (2011) Journal of internal medicine 270, 224-8. PMID: 21314738 5. Gin, P. et al. (2007) Biochimica et biophysica acta 1771, 1464-8. PMID: 17997385 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Human GPIHBP1 Protein, N-His-SUMO



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