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| カタログ番号 | HY489052 |
|---|---|
| 説明 |
Recombinant Human GRP78/HSPA5 Protein, N-His (HY489052) expressed in E. coli, spanning Glu25-Leu654. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P11021 |
| タンパク質長 | Glu25-Leu654 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 71.96 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Heat shock protein 70 family protein 5, 78 kDa glucose-regulated protein, HSPA5, Binding-immunoglobulin protein, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Endoplasmic reticulum chaperone BiP, GRP78, BiP, Immunoglobulin heavy chain-binding protein |
| 背景 | Endoplasmic reticulum chaperone BiP (HSPA5/GRP78) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells. 1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010 2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672 3. Evensen, NA. et al. (2013) Journal of the National Cancer Institute 105, 1402-16. PMID: 23990668 4. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555 5. Ma, K. et al. (2002) The Journal of biological chemistry 277, 18728-35. PMID: 11907036 6. Ng, DT. et al. (1992) Molecular biology of the cell 3, 143-55. PMID: 1550958 7. Oikawa, D. et al. (2009) Experimental cell research 315, 2496-504. PMID: 19538957 8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529 9. Kang, JM. et al. (2015) Cancer research 75, 3087-97. PMID: 26045166 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human GRP78/HSPA5 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
