Human GZMK Recombinant Protein (N-His) (HW458012)

Name: Human GZMK Recombinant Protein (N-His)
Brand: abinScience
SKU: HW458012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HW458012
Описание	Recombinant Human GZMK Protein, N-His (HW458012) expressed in E. coli, spanning Ile27-Asn264. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P49863
Длина белка	Ile27-Asn264
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	28.14 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	NK-tryptase-2, Fragmentin-3, Granzyme K, GZMK, Granzyme-3, TRYP2, NK-Tryp-2
Фон	Granzyme K (GZMK) is a ~28 kDa protein. Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway. First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization. It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway. GZMK-mediated complement activation is an important contributor to tissue inflammation. 1. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682 2. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882 3. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456 4. Wensink, AC. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 5974-9. PMID: 24711407 5. Mogilenko, DA. et al. (2021) Immunity 54, 99-115.e12. PMID: 33271118 6. Jonsson, AH. et al. (2022) Science translational medicine 14, eabo0686. PMID: 35704599
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human GZMK Protein, N-His

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Даташит

Human GZMK Recombinant Protein (N-His).pdf

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Formula

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