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Обзор
Каталожный номерHD437012
Описание
Recombinant Human HAT1 Protein, N-His (HD437012) expressed in E. coli, spanning Met1-Glu419. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаO14929
Длина белкаMet1-Glu419
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 51.68 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEC:2.3.1.48, HAT1, Histone acetyltransferase 1, Histone acetyltransferase type B catalytic subunit, KAT1
Фон

Histone acetyltransferase type B catalytic subunit is a ~49 kDa protein. Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression.

1. Campos, EI. et al. (2010) Nature structural & molecular biology 17, 1343-51. PMID: 20953179
2. Yang, X. et al. (2013) The Journal of biological chemistry 288, 18271-82. PMID: 23653357
3. Gruber, JJ. et al. (2019) Molecular cell 75, 711-724.e5. PMID: 31278053
4. Agudelo Garcia, PA. et al. (2020) Journal of proteome research 19, 1663-1673. PMID: 32081014
5. Makowski, AM. et al. (2001) The Journal of biological chemistry 276, 43499-502. PMID: 11585814
6. Wu, H. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 8925-30. PMID: 22615379
7. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 31812350
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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