Anti-HAT1 Polyclonal Antibody (HD437014)

Name: Anti-HAT1 Polyclonal Antibody
Brand: abinScience
SKU: HD437014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD437014

Description

Anti-HAT1 Polyclonal Antibody (HD437014) is a rabbit polyclonal antibody detecting HAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HAT1 (Met1-Glu419).

Target

EC:2.3.1.48, HAT1, Histone acetyltransferase 1, Histone acetyltransferase type B catalytic subunit, KAT1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O14929

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

O14929

Background

Histone acetyltransferase type B catalytic subunit (HAT1) is a ~49 kDa protein. Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression.

1. Campos, EI. et al. (2010) Nature structural & molecular biology 17, 1343-51. PMID: 20953179

2. Yang, X. et al. (2013) The Journal of biological chemistry 288, 18271-82. PMID: 23653357

3. Gruber, JJ. et al. (2019) Molecular cell 75, 711-724.e5. PMID: 31278053

4. Agudelo Garcia, PA. et al. (2020) Journal of proteome research 19, 1663-1673. PMID: 32081014

5. Makowski, AM. et al. (2001) The Journal of biological chemistry 276, 43499-502. PMID: 11585814

6. Wu, H. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 8925-30. PMID: 22615379

7. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 31812350

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-HAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: Lncap cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-12 cell lysate

References

Datasheet

Anti-HAT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HAT1 Polyclonal Antibody (HD437014)

Overview

Images

References

Datasheet

SDS

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