Human HMGB2 Recombinant Protein (N-His) (HB755012)

Name: Human HMGB2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB755012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HB755012
Описание	Recombinant Human HMGB2 Protein, N-His (HB755012) expressed in E. coli, spanning Gly2-Pro187. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P26583
Длина белка	Gly2-Pro187
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	23.43 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	HMGB2, High mobility group protein 2, HMG-2, HMG2, High mobility group protein B2
Фон	High mobility group protein B2 (HMGB2) is a ~24 kDa protein. Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity. 1. Fan, Z. et al. (2002) Molecular and cellular biology 22, 2810-20. PMID: 11909973 2. Zimmerman, J. et al. (2008) Biochemical and biophysical research communications 371, 79-84. PMID: 18413230 3. Ugrinova, I. et al. (2009) Biochemistry 48, 6502-7. PMID: 19522541 4. Laurent, B. et al. (2010) Blood 115, 687-95. PMID: 19965638 5. Stros, M. (2010) Biochimica et biophysica acta 1799, 101-13. PMID: 20123072 6. Shykind, BM. et al. (1995) Genes & development 9, 1354-65. PMID: 7797075 7. Pusterla, T. et al. (2009) Autoimmunity 42, 308-10. PMID: 19811285 8. Küchler, R. et al. (2013) Antimicrobial agents and chemotherapy 57, 4782-93. PMID: 23877675
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human HMGB2 Protein, N-His

Ссылки

Даташит

Human HMGB2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

C₁ (Stock Conc.)

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C₂ (Working Conc.)

V₂ (Working Vol.)

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