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| カタログ番号 | HY426012 |
|---|---|
| 説明 |
Recombinant Human IFIT1 Protein, N-His (HY426012) expressed in E. coli, spanning Met1-Pro478. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P09914 |
| タンパク質長 | Met1-Pro478 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 57.52 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | IFI-56K, G10P1, Interferon-induced protein with tetratricopeptide repeats 1, IFIT-1, IFNAI1, IFIT1, ISG56, IFI56, Interferon-induced 56 kDa protein, P56 |
| 背景 | Antiviral innate immune response effector IFIT1 is a ~55 kDa protein. Plays a key role in the innate immune response as part of an interferon-dependent multiprotein complex, recognizing and sequestering viral RNAs that lack host-specific 2'-O-methylation at their 5' cap. By distinguishing these RNAs from host mRNAs, inhibits their translation by competing with the translation initiation factor eIF4E. Could also prevent viral replication through its interaction with DNA replication origin-binding protein E1 of several viruses. Causes the translocation of E1 from the nucleus to the cytoplasm and can also inhibit its helicase activity in vitro. Exhibits antiviral activity against many viruses from the Flaviviridae (West Nile virus, Dengue virus, hepatitis C virus), Coronaviridae (human 229E coronavirus, SARS-CoV-2 and SARS-CoV), Poxviridae (vaccinia virus) and Togaviridae (Sindbis virus) families. 1. Pichlmair, A. et al. (2011) Nature immunology 12, 624-30. PMID: 21642987 2. Daugherty, MD. et al. (2016) eLife 5. PMID: 27240734 3. Geng, J. et al. (2024) RNA (New York, N.Y.) 30, 1292-1305. PMID: 39009378 4. Abbas, YM. et al. (2013) Nature 494, 60-4. PMID: 23334420 5. Abbas, YM. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2106-E2115. PMID: 28251928 6. Russ, A. et al. (2022) EMBO reports 23, e55648. PMID: 36285486 7. Terenzi, F. et al. (2008) The EMBO journal 27, 3311-21. PMID: 19008854 8. Raychoudhuri, A. et al. (2011) Journal of virology 85, 12881-9. PMID: 21976647 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human IFIT1 Protein, N-His
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