Human IFIT5 Recombinant Protein (N-His) (HC576012)

Name: Human IFIT5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC576012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HC576012
Описание	Recombinant Human IFIT5 Protein, N-His (HC576012) expressed in E. coli, spanning Met1-Ile482. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q13325
Длина белка	Met1-Ile482
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	58.01 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	ISG58, RI58, IFIT-5, P58, Interferon-induced protein with tetratricopeptide repeats 5, IFIT5, Retinoic acid- and interferon-inducible 58 kDa protein, Interferon-induced 58 kDa protein
Фон	Interferon-induced protein with tetratricopeptide repeats 5 (IFIT5) is a ~55 kDa protein. Interferon-induced RNA-binding protein involved in the human innate immune response. Has a broad and adaptable RNA structure recognition important for RNA recognition specificity in antiviral defense. Binds precursor and processed tRNAs as well as poly-U-tailed tRNA fragments. Specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. 1. Katibah, GE. et al. (2013) Molecular cell 49, 743-50. PMID: 23317505 2. Feng, F. et al. (2013) Cell research 23, 1055-8. PMID: 23774268 3. Katibah, GE. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 12025-30. PMID: 25092312 4. Abbas, YM. et al. (2013) Nature 494, 60-4. PMID: 23334420 5. Zheng, C. et al. (2015) Cellular signalling 27, 2343-54. PMID: 26334375
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human IFIT5 Protein, N-His

Ссылки

Даташит

Human IFIT5 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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