ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Applications testées
WB
Cible
IL-13, IL13, Interleukin-13, NC30
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
14.55 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
IL-13, IL13, Interleukin-13, NC30
Contexte
Interleukin-13 (IL13) is a ~15 kDa protein. Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B.
1. Minty, A. et al. (1993) Nature 362, 248-50. PMID: 8096327
2. McKenzie, AN. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 3735-9. PMID: 8097324
3. Defrance, T. et al. (1994) The Journal of experimental medicine 179, 135-43. PMID: 7903680
4. Luttmann, W. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 1678-83. PMID: 8759755
5. Schnyder, B. et al. (1996) Blood 87, 4286-95. PMID: 8639787
6. Miloux, B. et al. (1997) FEBS letters 401, 163-6. PMID: 9013879
7. Kasaian, MT. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 187, 561-9. PMID: 21622864
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human IL13 Protein, N-His
Bioactivity
Detected by Research Grade Anrukinzumab (Cat No.: HW632066) inindirectELISA.
Western blot
Detected by Anti-IL13 monoclonal antibody (Catalog No: HW632066)
Western blot
Detected by Anti-IL13 monoclonal antibody (Catalog No: HS882207)
Western blot
Detected by Anti-IL13 monoclonal antibody (Catalog No: HW632107)
Western blot
Detected by Anti-IL13 monoclonal antibody (Catalog No: HB978016)
Western blot
Detected by Anti-IL13 monoclonal antibody (Catalog No: HW632010)
Bioactivity
Detected by Donzakimig (Catalog No: HV999026) in indirect ELISA.
Bioactivity
Detected by Galvokimig (Catalog No: HS856206) in indirect ELISA.
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