Human IL-15 Recombinant Protein (C-His) (HW541012)

Name: Human IL-15 Recombinant Protein (C-His)
Brand: abinScience
SKU: HW541012
Price: 211 USD
Availability: InStock

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1mg

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Número de catálogo	HW541012
Descripción	Recombinant Human IL15 Protein, C-His (HW541012) expressed in E. coli, spanning Asn49-Ser162. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P40933
Longitud de proteína	Asn49-Ser162
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	14.03 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	IL15, IL-15, Interleukin-15
Fondo	Interleukin-15 (IL15) is a ~18 kDa protein. Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. 1. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770 2. Grabstein, KH. et al. (1994) Science (New York, N.Y.) 264, 965-8. PMID: 8178155 3. Badolato, R. et al. (1997) Blood 90, 2804-9. PMID: 9326248 4. Musso, T. et al. (1999) Blood 93, 3531-9. PMID: 10233906 5. Ring, AM. et al. (2012) Nature immunology 13, 1187-95. PMID: 23104097 6. Giri, JG. et al. (1994) The EMBO journal 13, 2822-30. PMID: 8026467 7. Johnston, JA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8705-9. PMID: 7568001
Nota	For research use only.