Human IL-4 Recombinant Protein (N-Fc) (HY457011)

Name: Human IL-4 Recombinant Protein (N-Fc)
Brand: abinScience
SKU: HY457011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY457011
説明	Recombinant Human IL4 Protein, N-Fc (HY457011) expressed in Mammalian Cells, spanning His25-Ser153. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	Mammalian cells
アクセッション番号	P05112
タンパク質長	His25-Ser153
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4
背景	Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895 _x005F_x005F_x000D_
注意事項	For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Recombinant Human IL4 Protein, N-Fc
Bioactivity

Immobilized HY457011 Human IL4 Recombinant Protein (N-Fc) at 2 μg/mL(100 μL/well) can bind HB732011 Human CD124/IL4R Recombinant Protein (C-His), the EC 50 is 10.2 ng/mL.
Bioactivity

Immobilized HB732011 Human CD124/IL4R Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HY457011 Human IL4 Recombinant Protein (N-Fc), the EC 50 is 16.5 ng/mL.