Anti-Human IL-4 Reference Antibody (Vak-694, RUO) (HY457026)

Name: Anti-Human IL-4 Reference Antibody (Vak-694, RUO)
Brand: abinScience
SKU: HY457026
Price: 326 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY457026

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Host species

Human

Isotype

IgG1

Biological activity

VAK-694 binds to IL-4, reduces allergen-specific IL-4 producing cell numbers [1]. VAK-694 exerts immunomodulatory effects on TH2 memory cells [2].

Expression system

Mammalian cells

Clonality

Monoclonal

Tested applications

Target

Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05112

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

VAK-694, NVP-VAK-694

Background

Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171

2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727

3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231

4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247

5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337

6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101

7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895

References

1. Koneczny I, et al. IgG4 Autoantibodies in Organ-Specific Autoimmunopathies: Reviewing Class Switching, Antibody-Producing Cells, and Specific Immunotherapies. Front Immunol. 2022;13:834342. Published 2022 Mar 24. [HY457026]
2. Pfaar O, et al. Anti-IgE: A treatment option in allergic rhinitis?. Allergol Select. 2021;5:119-127. Published 2021 Feb 24. [HY457026]

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade VAK-694.
Bioactivity

Detects recombinant IL4 (Catalog No: HY457021) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-IL4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL4 (Catalog No: HY457021)
Lane 2: Negative control