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| カタログ番号 | HC502012 |
|---|---|
| 説明 |
Recombinant Human ILK Protein, N-His (HC502012) expressed in E. coli, spanning Asn183-Lys452. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13418 |
| タンパク質長 | Asn183-Lys452 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.12 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ILK, ILK2, ILK1, ILK-2, ILK-1, 59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, p59ILK, Integrin-linked protein kinase |
| 背景 | Scaffold protein ILK is a ~51 kDa protein. Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes. 1. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447 2. Fukuda, K. et al. (2009) Molecular cell 36, 819-30. PMID: 20005845 3. Vaynberg, J. et al. (2018) Nature communications 9, 4465. PMID: 30367047 4. Yang, L. et al. (2020) Nature 583, 133-138. PMID: 32528174 5. Yoshimi, R. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 3611-24. PMID: 16517730 6. Delcommenne, M. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11211-6. PMID: 9736715 7. Fielding, AB. et al. (2008) The Journal of cell biology 180, 681-9. PMID: 18283114 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ILK Protein, N-His
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