Anti-ILK Polyclonal Antibody (HC502014)

Name: Anti-ILK Polyclonal Antibody
Brand: abinScience
SKU: HC502014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC502014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ILK (Asn183-Lys452).

Target

ILK, ILK2, ILK1, ILK-2, ILK-1, 59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, p59ILK, Integrin-linked protein kinase

Purification

Purified by antigen affinity column.

Accession

Q13418

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Scaffold protein ILK is a ~51 kDa protein. Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes.

1. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

2. Fukuda, K. et al. (2009) Molecular cell 36, 819-30. PMID: 20005845

3. Vaynberg, J. et al. (2018) Nature communications 9, 4465. PMID: 30367047

4. Yang, L. et al. (2020) Nature 583, 133-138. PMID: 32528174

5. Yoshimi, R. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 3611-24. PMID: 16517730

6. Delcommenne, M. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11211-6. PMID: 9736715

7. Fielding, AB. et al. (2008) The Journal of cell biology 180, 681-9. PMID: 18283114

Note

For research use only.

Images

References

Datasheet

Anti-ILK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-ILK Polyclonal Antibody (HC502014)

Overview

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References

Datasheet

SDS

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