Human IRS2 Recombinant Protein (N-His) (HV817032)

Name: Human IRS2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV817032
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HV817032
Description	Recombinant Human IRS2 Protein, N-His (HV817032) expressed in E. coli, spanning Gly185-Arg301. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9Y4H2
Longueur de protéine	Gly185-Arg301
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	15.31 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	IRS-2, IRS2, Insulin receptor substrate 2
Contexte	Insulin receptor substrate 2 is a ~137 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. 1. Fukushima, T. et al. (2015) Nature communications 6, 6780. PMID: 25879670 2. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100 3. Duan, C. et al. (2004) The Journal of biological chemistry 279, 43684-91. PMID: 15316008 4. Heller, NM. et al. (2008) Science signaling 1, ra17. PMID: 19109239 5. Manohar, S. et al. (2020) Molecular & cellular proteomics : MCP 19, 1450-1467. PMID: 32554797
Note	For research use only

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Human IRS2 Recombinant Protein (N-His).pdf

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