Anti-IRS2 Polyclonal Antibody (HV817014)

Name: Anti-IRS2 Polyclonal Antibody
Brand: abinScience
SKU: HV817014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HV817014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IRS2 (Gly185-Arg301).

Target

IRS2, Insulin receptor substrate 2, IRS-2

Purification

Purified by antigen affinity column.

Accession

Q9Y4H2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Insulin receptor substrate 2 (IRS2) is a ~137 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation.

1. Fukushima, T. et al. (2015) Nature communications 6, 6780. PMID: 25879670

2. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100

3. Duan, C. et al. (2004) The Journal of biological chemistry 279, 43684-91. PMID: 15316008

4. Heller, NM. et al. (2008) Science signaling 1, ra17. PMID: 19109239

5. Manohar, S. et al. (2020) Molecular & cellular proteomics : MCP 19, 1450-1467. PMID: 32554797

Note

For research use only.

Images

References

Datasheet

Anti-IRS2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-IRS2 Polyclonal Antibody (HV817014)

Overview

Images

References

Datasheet

SDS

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