ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
16.30 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
KiSS-1 receptor is a ~42 kDa protein. Receptor for kisspeptins (kisspeptin-10, kisspeptin-13, kisspeptin-14 and metastin/kisspeptin-54). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.
1. Kotani, M. et al. (2001) The Journal of biological chemistry 276, 34631-6. PMID: 11457843
2. Hori, A. et al. (2001) Biochemical and biophysical research communications 286, 958-63. PMID: 11527393
3. Bilban, M. et al. (2004) Journal of cell science 117, 1319-28. PMID: 15020672
4. Becker, JA. et al. (2005) Biochemical and biophysical research communications 326, 677-86. PMID: 15596153
5. de Roux, N. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 10972-6. PMID: 12944565
6. Seminara, SB. et al. (2003) The New England journal of medicine 349, 1614-27. PMID: 14573733
7. Semple, RK. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 1849-55. PMID: 15598687
8. Tenenbaum-Rakover, Y. et al. (2007) The Journal of clinical endocrinology and metabolism 92, 1137-44. PMID: 17164310
9. Teles, MG. et al. (2008) The New England journal of medicine 358, 709-15. PMID: 18272894
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Human KISS1R Protein, N-His-SUMO
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