Anti-KISS1R Polyclonal Antibody (HV546014)

Name: Anti-KISS1R Polyclonal Antibody
Brand: abinScience
SKU: HV546014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HV546014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KISS1R (His2-Trp43).

Target

KiSS-1R, G-protein coupled receptor 54, KiSS-1 receptor, GPR54, KISS1R, hOT7T175, G-protein coupled receptor OT7T175, Metastin receptor, Hypogonadotropin-1, AXOR12, Kisspeptins receptor

Purification

Purified by antigen affinity column.

Accession

Q969F8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

KiSS-1 receptor (KISS1R) is a ~42 kDa protein. Receptor for kisspeptins (kisspeptin-10, kisspeptin-13, kisspeptin-14 and metastin/kisspeptin-54). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.

1. Kotani, M. et al. (2001) The Journal of biological chemistry 276, 34631-6. PMID: 11457843

2. Hori, A. et al. (2001) Biochemical and biophysical research communications 286, 958-63. PMID: 11527393

3. Bilban, M. et al. (2004) Journal of cell science 117, 1319-28. PMID: 15020672

4. Becker, JA. et al. (2005) Biochemical and biophysical research communications 326, 677-86. PMID: 15596153

5. de Roux, N. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 10972-6. PMID: 12944565

6. Seminara, SB. et al. (2003) The New England journal of medicine 349, 1614-27. PMID: 14573733

7. Semple, RK. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 1849-55. PMID: 15598687

8. Tenenbaum-Rakover, Y. et al. (2007) The Journal of clinical endocrinology and metabolism 92, 1137-44. PMID: 17164310

9. Teles, MG. et al. (2008) The New England journal of medicine 358, 709-15. PMID: 18272894

Note

For research use only.

Images

References

Datasheet

Anti-KISS1R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-KISS1R Polyclonal Antibody (HV546014)

Overview

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References

Datasheet

SDS

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