Human KLHL3 Recombinant Protein (N-His) (HA118012)

Name: Human KLHL3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA118012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HA118012
Descripción	Recombinant Human KLHL3 Protein, N-His (HA118012) expressed in E. coli, spanning Thr27-Lys292. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9UH77
Longitud de proteína	Thr27-Lys292
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	32.59 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Kelch-like protein 3, KLHL3, KIAA1129
Fondo	Kelch-like protein 3 (KLHL3) is a ~64 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation. 1. Furukawa, M. et al. (2003) Nature cell biology 5, 1001-7. PMID: 14528312 2. Louis-Dit-Picard, H. et al. (2012) Nature genetics 44, 456-60, S1-3. PMID: 22406640 3. Ohta, A. et al. (2013) The Biochemical journal 451, 111-22. PMID: 23387299 4. Wakabayashi, M. et al. (2013) Cell reports 3, 858-68. PMID: 23453970 5. Shibata, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7838-43. PMID: 23576762 6. Wu, G. et al. (2013) FEBS letters 587, 1717-22. PMID: 23665031 7. Shibata, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 15556-61. PMID: 25313067 8. Etges, A. et al. (2022) Nephron 146, 418-428. PMID: 35093948
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human KLHL3 Protein, N-His

Referencias

Datasheet

Human KLHL3 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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